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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Levofloxacin, a synthetic fluoroquinolone, is an antibacterial agent that inhibits the supercoiling activity of bacterial DNA gyrase, halting DNA replication.
Cell lines
Osteoblastic cells
Preparation method
The solubility of this compound in DMSO is ≥11.4 mg/ml. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
Reacting condition
80 μg/ml
Applications
Levofloxacin has the smallest inhibitory effect on osteoblast growth, with a concentration of approximately 80 mg / mL at 50% inhibition at 48 and 72 hours. Levofloxacin strongly inhibits calcium deposition, as can be determined by alizarin red staining and biochemical analysis on day 14.
Animal models
Juvenile New Zealand White rabbits
Dosage form
100 mg/kg (p.o.), for 7 days
Application
Levofloxacin initially inhibited the synthesis of glycosaminoglycans, followed by the inhibition of DNA synthesis and mitochondrial function in cultured rabbit chondrocytes at actual arthritic concentrations, but these changes were reversible and were not sufficient to kill the cells.
Other notes
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References:
[1]. Holtom PD, Pavkovic SA, Bravos PD, Patzakis MJ, Shepherd LE, Frenkel B.Inhibitory effects of the quinolone antibiotics trovafloxacin, ciprofloxacin, and levofloxacin on osteoblastic cells in vitro. J Orthop Res. 2000 Sep;18(5):721-7. PubMed PMID: 11117292.
[2]. Kato M, Takada S, Ogawara S, Takayama S. Effect of levofloxacin on glycosaminoglycan and DNA synthesis of cultured rabbit chondrocytes at concentrations inducing cartilage lesions in vivo. Antimicrob Agents Chemother.1995 Sep;39(9):1979-83. PubMed PMID: 8540702; PubMed Central PMCID: PMC162867.