2X HyperFusion™ High-Fidelity Master Mix (With dye)
Our product 2X HyperFusion™ High-Fidelity Master Mix (With dye) offers superior performance for major PCR applications with high fidelity, powerful amplification ability and faster amplification speed. HyperFusion™ high-fidelity DNA polymerases are composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. The high fidelity makes HyperFusion™ high-fidelity DNA polymerase a superior choice for detection or other subsequent applications. HyperFusion™ high-fidelity DNA polymerase possesses one of the most accuracy with an error rate that is 50-fold lower compared to Taq DNA Polymerase and 6-fold lower than Pyrococcus Furiosus DNA Polymerase (Pfu).
HyperFusion™ DNA polymerase possesses 5´→ 3´ polymerase activity and 3´→ 5´ exonuclease activity. It will generate blunt-ended products in the amplification products without an A overhang which appears in the product amplified with Taq polymerase. The polymerase has been capable of amplifying fragment as long as 10 kb in our assays. In PCR reaction, elongation rate of HyperFusion™ DNA polymerase is about 15-30 sec/kb depending on the complexity of the gene.
2X HyperFusion™ High-Fidelity Master Mix (With dye) is a ready-to-use 2X premix solution containing polymerase, dNTPs, an already optimized buffer system and dye. Our mix is ideal for any detection and the PCR product can be directly electrophoresed after the amplification without the need to add a loading buffer.
If the subsequent experiment is cloning, product 2X HyperFusion™ High-Fidelity Master Mix (Cat No. K1039) is your superior choice which doesn’t contain a dye.
| Component | Size |
| 2X HyperFusion™ High-Fidelity Master Mix (With dye) | 1 mL |
| 2X HyperFusion™ High-Fidelity Master Mix (With dye) | 5X1 mL |
| 2X HyperFusion™ High-Fidelity Master Mix (With dye) | 10X1 mL |
Store the master mix at -20°C. | |
High fidelity —up to 50X more accurate than Taq; up to 6X more accurate than Pfu DNA polymerase.
Enhanced robustness—fewer reaction failures and minimal optimization.
Improved yields—high product yields in one reaction.
High speed—increased processivity allows shorter reaction times.
Quick and simply ready-to-use PCR mix.
