4-Thiouridine
4-Thiouridine is a photoactivatable ribonucleoside analog [1].
4-Thiouridine at high concentrations (> 100 µM) is used for various RNA analyses such as photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation, as well as nascent messenger RNA (mRNA) labeling [1].
In 2fTGH cells, 4-thiouridine at low concentrations ≤ 10 µM could be used to measure production and processing of ribosomal RNA (rRNA). However, in U2OS cells, elevated concentrations of 4-thiouridine (> 50 µM), commonly used for mRNA labeling experiments, inhibited production and processing of 47S rRNA, which was accompanied by induction of tumor suppressor p53, nucleoplasmic translocation of nucleolar nucleophosmin, and inhibition of cell proliferation [1].
In an in vivo model of Sephadex (SDX)-induced lung inflammation with Sprague–Dawley rats, 4-thiouridine (50 mg /kg, via intratracheal instillation) caused a 38.5% reduction of lung edema. Co-administration of 4-thiouridine at 50 mg /kg reduced total leukocyte count by 58%, and led to reductions in all leukocyte groups [2].
References:
[1]. Burger K, Mühl B, Kellner M, et al. 4-Thiouridine inhibits rRNA synthesis and causes a nucleolar stress response. RNA Biology, 2013, 10(10): 1623-1630.
[2]. Uppugunduri S, Gautam C. Effects of uridine, isomatitol and 4-thiouridine on in vitro cell adhesion and in vivo effects of 4-thiouridine in a lung inflammation model. International Immunopharmacology, 2004, 4(9): 1241-1248.
- 1. Dan Zhao, Lu Zhang, et al. "Transcriptome dynamics in mouse amygdala under acute and chronic stress revealed by thiol-labeled RNA sequencing." Neurobiol Stress. 2024 Nov 7:33:100688
- 2. Haoqing Yang, Wanqing Wang, et al. "miR615‐3p inhibited FBLN1 and osteogenic differentiation of umbilical cord mesenchymal stem cells by associated with YTHDF2 in a m6A‐miRNA interaction manner." Cell Prolif. 2024 Jun;57(6):e13607 PMID: 38353178
- 3. Luyuan Jin, et al. "miR615-3p inhibited FBLN1 and osteogenic differentiation of umbilical cord mesenchymal stem cells by associated with YTHDF2 in a m6A-miRNA interaction manner." Cell Prolif. 2024 Feb 14:e13607. PMID: 38353178
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 260.26 |
| Cas No. | 13957-31-8 |
| Formula | C9H12N2O5S |
| Solubility | ≥107.8 mg/mL in DMSO; ≥25.85 mg/mL in EtOH; ≥44.8 mg/mL in H2O |
| Chemical Name | 1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-4-thioxo-3,4-dihydropyrimidin-2(1H)-one |
| SDF | Download SDF |
| Canonical SMILES | OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C=CC(NC2=O)=S)O1 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
