5-Methyl-dCTP
5-Methyl-dCTP is a base modified deoxynucleotide. Methylation of the C-5 position of cytosines is a common and important epigenetic modification, with more than 4% of the cytosines present in the human genome reported to be methylated. This modification can be incorporated into DNA via the polymerase chain reaction (PCR) with 5-methyl-dCTP instead of dCTP. The ability to synthesize a defined, fully 5-methyl-dCTP-substituted DNA by PCR will be a valuable resource for a variety of applications including testing restriction endonucleases for methyl sensitivity and determining the specificity of 5-methyl-dCTP-dependent endonucleases. In addition, electroporation of 5-methyl-dCTP into living cells can also be incorporated into genomic DNA, and heritably alter cytosine methylation patterns and gene expression.
References:
1. Kumar S, Chinnusamy V, Mohapatra T. Epigenetics of Modified DNA Bases: 5-Methylcytosine and Beyond. Frontiers in Genetics, 2018, 9: 640.
2. Wong KK, McClelland M. PCR with 5-methyl-dCTP replacing dCTP. Nucleic Acids Research, 1991, 19(5): 1081-1085.
3. Lao VV, Herring JL, Kim CH, Darwanto A, Soto U, Sowers LC. Incorporation of 5-chlorocytosine into mammalian DNA results in heritable gene silencing and altered cytosine methylation patterns. Carcinogenesis, 2009, 30(5): 886-893.
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 481.1 (free acid) |
| Formula | C10H18N3O13P3 (free acid) |
| Synonyms | 5-Methyl-2'-deoxycytidine-5'-Triphosphate, m5-dCTP, 5-m-dCTP, 5m-dCTP |
| Chemical Name | lithium (5-(4-amino-5-methyl-2-oxopyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl triphosphate |
| SDF | Download SDF |
| Canonical SMILES | NC(C(C)=CN1C2CC(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O2)=NC1=O.[Li+].[Li+].[Li+].[Li+] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
- View current batch:
-
Purity ≥95% by AX-HPLC
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
