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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The 7-AAD Cell Viability Assay Kit is a kit that uses the fluorescent probe 7-AAD to detect cell necrosis specifically. 7-AAD (7-amino actinomycin D) is a commonly used nuclear probe that binds to DNA and emits red fluorescence. Similar to propidium iodide (PI), 7-AAD is not a cell-permeable probe, so 7-AAD can only pass through cells with damaged membranes and stain their nuclei. But unlike PI, 7-AAD has a narrower emission spectrum, and longer emission wavelengths, leading to less interference to other channels. Therefore, 7-AAD is often used to replace PI in combination with other fluorescent dyes (such as FITC, PE, and APC).
This kit is suitable for the detection of apoptosis and necrosis in combination with Calcein AM, Annexin V-FITC, Annexin V-PE, and so on. This kit can be detected by fluorescence microscopy, flow cytometry or other fluorescence detection instruments after staining. This kit can also be used in flow cytometry to exclude nonviable cells.
Figure 1: The efficiency of this kit for detecting the proliferation of Jurkat cells in combination with Calcein AM Cell Viability Assay Kit (K2231)
This product should be stored at -20°C, stable for 1 year. 7-AAD (20X) should be stored at -20°C away from light, and avoid repeated freeze/thaw cycles.