JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.
Tel: +1-832-696-8203
Email: [email protected]
Worldwide Distributors
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ac-VEID-AFC is a fluorogenic substrate of caspase-6 (Km=30μM; Kcat/Km=168000 M-1sec-1) and related cysteine proteases. The sequence is based on the lamin A site of cleavage. During apoptosis, caspase-6 cleaves the substrate and release AFC which then can be quantified by fluorescent detection at Ex/Em = 400/505. Ac-IETD-pNA can be used to identify and quantify caspase-6 activity in apoptotic cell lysates and to study events downstream of caspase-6 activation.
References:
1. A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem. 272, 17907 (1997)
2. Substrate specificities of caspase family proteases: R.V. Talanian, et al.; J. Biol. Chem. 272, 9677 (1997)
3. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32: S.M. Srinivasula, et al.; J. Biol. Chem. 271, 27099 (1996)
