HyperFluor™ 488 Goat Anti-Rabbit IgG (H+L) Antibody
| Name | HyperFluor™ 488 Goat Anti-Rabbit IgG (H+L) Antibody |
| Host species | Goat |
| Target species | Rabbit |
| Tested applications | Suitable for: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISA |
| Conjugation | Alexa 488. Ex: 495 nm, Em: 519 nm |
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Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for Rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
| Application | Notes |
| ICC/IF | 1/500-1/2000 |
| IHC-Fr | Use at an assay dependent dilution. |
| IHC-P | 1/100-1/500 |
| Flow Cyt | 1/250-1/1000 |
| ELISA | Use at an assay dependent dilution. |
| Form | Liquid |
| Storage buffer | Preservative: 0.02% Sodium azide Constituents: 23% Glycerol, PBS, 1% BSA |
| Storage instructions | Shipped at 4°C. Store at 4°C short term (2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Keep away from Light. |
| Concentration | 1 mg/mL |
| Purity | Immunogen affinity purified |
| Purification notes | This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. |
| Clonality | Polyclonal |
| Isotype | IgG |