Alkaline Phosphatase Activity Colorimetric Assay Kit

Alkaline phosphatase (ALP) is a hydrolase enzyme that removes phosphate groups from nucleotides proteins and alkaloids. Changes in ALP level and activity are associated with various disease states in the bone and liver.

 The Alkaline Phosphatase Activity Colorimetric Assay Kit provides a highly sensitive and convenient way for detection of ALP activity based on colorimetric method in serum and biological samples. The kit contains 10 substrate tablets providing convenience for multiple usages. The assay utilizes p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which produces yellow (λmax = 405 nm) when dephosphorylated by ALP that can be easily detected using a spectrophotometer or an ELISA plate reader. The assay can detect 10-250 µU ALP in various samples.