AO/PI Double Staining Kit
- 1. Dorota Ciołczyk-Wierzbicka, Marta Zarzycka, et al. "Treatment of Melanoma Cells with Chloroquine and Everolimus Activates the Apoptosis Process and Alters Lipid Redistribution." Int J Mol Sci. 2024 Nov 15;25(22):12278. PMID: 39596342
- 2. Hui-Da Li, Yuan-Qiang Chen, et al. "Harnessing virus flexibility to selectively capture and profile rare circulating target cells for precise cancer subty." Nat Commun. 2024 Jul 12;15(1):5849. PMID: 38992001
AO/PI Double Staining Kit is a kit that can rapidly detect cell viability. This kit provides two dyes, Acridine Orange (AO) and propidium iodide (PI). AO can penetrate cell membranes, staining normal cells in green. Chromatin condensation is a hallmark of apoptosis. AO can stain condensed chromatin of apoptotic cells more brightly than normal cells. PI cannot penetrate the cell membrane and cannot stain normal cells or apoptotic cells red; for necrotic cells (whose membrane loses integrity), PI can stain them red. The staining pattern using these two dyes makes it possible to distinguish normal cells (green fluorescence), apoptotic cells (orange fluorescence) and necrotic cells (red fluorescence).
Figure 1: Diagram of this kit for detecting cell viability
| Components | K2238-100 T | K2238-500 T |
| AO staining solution | 500 μL | 5 x 500 μL |
| PI staining solution | 500 μL | 5 x 500 μL |
| 10X staining buffer | 1 mL | 5 x 1 mL |
Store the kit at -20°C, stable for 1 year. AO staining solution and PI staining solution need to be stored away from light. For frequent use, store the kit at 4°C. | ||