Biotin-XX Tyramide Reagent
Biotin-LC-LC-tyramide is used for tyramide signal amplification (TSA) which is a powerful, patented technology that significantly enhances both chromogenic and fluorescent signals. TSA is easily integrated into standard nonradioactive in situ hybridization (ISH) or IHC protocols.
TSA is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to catalyze the deposition of a fluorophore-labeled tyramide amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins.
Biotin-LC-LC-tyramide use horseradish peroxidase (HRP) to catalyze covalent deposition of biotin labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can be detected with streptavidin conjugates for imaging in brightfield or fluorescence microscopy.
- 1. Zora Chui-Kuen Chan, Cheng Qi, et al. "Serotonin-Induced Inhibition of HRP-Mediated Proximity Labelling." Research Square. 30 Dec, 2024
- 2. Pengzhen Dong, Yue Li, et al. "Fat body-derived cytokine Upd2 controls disciplined migration of tracheal stem cells in Drosophila." bioRxiv. November 12, 2024.
- 3. Carlos Pascual-Caro, Jaime de Juan-Sanz, et al. "Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling." PLoS Biol. 2024 Oct 28;22(10):e3002860. PMID: 39466808
- 4. Ulku Cuhadar, Lorenzo Calzado-Reyes, et al. "Activity-driven synaptic translocation of LGI1 controls excitatory neurotransmission." Cell Rep. 2024 May 28;43(5):114186. PMID: 38700985
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 589.79 |
| Formula | C30H47N5O5S |
| Synonyms | biotin-LC-LC-tyramide |
| Solubility | ≥59 mg/mL in DMSO; insoluble in H2O; ≥14.1 mg/mL in EtOH with ultrasonic |
| Chemical Name | N-(4-hydroxyphenethyl)-6-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)hexanamide |
| SDF | Download SDF |
| Canonical SMILES | O=C1N[C@@]([H])([C@]([H])(CS2)N1)[C@@H]2CCCCC(NCCCCCC(NCCCCCC(NCCC3=CC=C(O)C=C3)=O)=O)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
