Biotinylated Protein Pull-Down Kit (Magnetic Beads)

The Biotinylated Protein Pull - Down Kit (Magnetic Beads) is a kit for studying protein-protein interactions and related biomolecule-to-molecule interactions. It uses the high affinity between biotin and streptavidin to combine biotin-labeled proteins, antibodies or other active biomolecules with streptavidin magnetic beads and elute, capture the target protein or other binding molecules that interact with biotin-labeled target proteins and biotin-labeled proteins, and carry out pull-down experiments for biotinylated protein complexes, and acidic conditions are important conditions for eluting target molecules or complexes.

The kit provides reagents such as streptavidin magnetic beads for binding biotin tags on biomolecules such as tagged proteins, blocking solutions, acid washes, and eluents. It can be used to study the interaction between a known protein (bait protein) and a target protein to be discovered (prey protein) or between a known protein and DNA, RNA, or other molecules, as well as to confirm the interaction between a known protein and a biomolecule such as a protein. If this kit is used for pull down and elution of biotinylated protein complexes, the experimental workflow will be easier and more convenient. The eluate can be used to identify the bound protein of interest or other biomolecules by Western, mass spectrometry, PCR, DNA sequencing, and other analyses.