Blasticidin S HCl
Blasticidin S HCl is an antibiotic inhibiting protein synthesis [1].
Blasticidin S is isolated from Streptomyces griseochromogenes and inhibits protein synthesis in both prokaryotic and eukaryotic cells.
Blasticidin S HCl is an antibiotic inhibiting protein synthesis. In mouse leukemia L5178Y cells, blasticidin S inhibited protein synthesis and caused the degradation of presumptive mRNA [1]. Also, blasticidin S inhibited ribonuclease P activity from Dictyostelium discoideum and inhibited tRNA maturation in a dose dependent way [2]. In S. cerevisiae, blasticidin S inhibited protein synthesis induced by galactose. In Aspergillus parasiticus, blasticidin S inhibited aflatoxin production without inhibition of fungal growth. In Aspergillus flavus, blasticidin S significantly reduced aflatoxin biosynthetic enzymes [3]. Blasticidin S efficiently bound Cu2+ ions and protected DNA from metal-induced damage [4]. Blasticidin S enhanced the binding of tRNA to the P site of the large ribosomal subunit and slowed spontaneous intersubunit rotation. Blasticidin S inhibited peptide bond formation and peptidyl-tRNA hydrolysis [5].
References:
[1] Takenaka K, Endo H, Kuwano M. The synthesis and degradation of presumptive messenger RNA in cultured mouse leukemia cells during the inhibition of protein synthesis. Biochim Biophys Acta, 1978, 521 (1): 295-307.
[2] Stathopoulos C, Tsagla A, Tekos A, et al. Effect of peptidyltransferase inhibitors on ribonuclease P activity from Dictyostelium discoideum. Effect of antibiotics on RNase P. Mol Biol Rep, 2000, 27 (2): 107-111.
[3] Yoshinari T, Noda Y, Yoda K, et al. Inhibitory activity of blasticidin A, a strong aflatoxin production inhibitor, on protein synthesis of yeast: selective inhibition of aflatoxin production by protein synthesis inhibitors. J Antibiot (Tokyo), 2010, 63 (6): 309-314.
[4] Stokowa-Sołtys K, Jeżowska-Bojczuk M. A rice fungicide blasticidin S efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. J Inorg Biochem, 2013, 127: 73-78.
[5] Svidritskiy E, Ling C, Ermolenko DN, et al. Blasticidin S inhibits translation by trapping deformed tRNA on the ribosome. Proc Natl Acad Sci U S A, 2013, 110 (30): 12283-12288.
| Physical Appearance | White solid |
| Storage | Store at -20°C |
| M.Wt | 458.9 |
| Cas No. | 3513-03-9 |
| Formula | C17H27ClN8O5 |
| Solubility | insoluble in DMSO; insoluble in EtOH; ≥52.4 mg/mL in H2O |
| Chemical Name | (2S,3S,6R)-3-[[(3R)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2H-pyran-2-carboxylic acid;hydrochloride |
| SDF | Download SDF |
| Canonical SMILES | CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
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Cell lines |
Mouse leukemia L5178Y cells |
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Reaction Conditions |
20 μg/ml blasticidin S for 6 h incubation |
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Applications |
In mouse leukemia L5178Y cells, blasticidin S resulted in a marked accumulation of monosome and ribosomal subunits, accompanied by a drastic decrease of pulse-labeled RNA (presumably mRNA) in the larger size region of polysomes. In response to a decreased protein synthesis (5 ~ 10% of the control), the appearance of pulse-labeled [3H]RNA among polysomes and monosome decreased to approximately 10% of the control when cells were treated with blasticidin S. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Takenaka K, Endo H, Kuwano M. The synthesis and degradation of presumptive messenger RNA in cultured mouse leukemia cells during the inhibition of protein synthesis. Biochimica et Biophysica Acta, 1978, 521 (1): 295-307. |
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Quality Control & MSDS
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Chemical structure
