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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cysteine-containing aspartate proteolytic enzymes (Caspase) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Caspase-2 is considered to be an upstream initiator of apoptosis signaling, and activation of caspase-2 can trigger a series of downstream events, including activation of executor caspases (e.g., caspase-3, caspase-7), thereby promoting the execution of apoptosis.
Caspase-2 Colorimetric Assay Kit provides a convenient and simple method for the detection of VDVAD-dependent caspase activity. When caspase-2 or related caspases cleave VDVAD from the labeled substrate VDVAD-p-nitroaniline (VDVAD-pNA), the light emission of free pNA can be measured at 405 nm or 400 nm using a microplate reader or spectrophotometer. The absorbance of free pNA in apoptotic samples was compared to an uninduced control to determine a fold increase in caspase-2 activity.
Figure 1: Reference standard curve for this kit
K2017-25T
K2017-50T
K2017-100T
Reagent I
20 mL
25 mL
Reagent II
30 mL
60 mL
120 mL
Reagent III
0.25 mL
0.55 mL
2 X 0.55 mL
pNA Standard (5 mM)
1 mL
Store the kit at -20°C, stable for 6 months. Reagent III and pNA Standard (5 mM) should be stored away from light.