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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell proliferation can be detected by a variety of methods. Cell proliferation induces an increase in the activity of the mitochondrial dehydrogenases, which cleaves the tetrazolium salt to formazan.
The WST Cell Proliferation Colorimetric Assay Kit plus provides the easiest and most sensitive way for quantification of cell proliferation and viability. Cell proliferation causes the increase in the amount of formazan dye formed that is directly proportional to the number of living cells and can be quantified by measuring the absorbance of the dye solution at 440 nm using microtiter plate reader. The kit can be used for the analysis of cell proliferation in response to pharmaceutical compounds, cytotoxic compounds like anticancer drugs and many other toxic agents. The assay can also be used for the detection of cell proliferation in response to cytokines, mitogens, growth factors and nutrients, etc. The assay is very easy and simple, just add-and-read, requiring no solubilization, no washing and no harvesting steps, and is stable, faster and more sensitive than MTS, MTT or XTT-based assays. The entire assay can be performed in a microtiter plate and correlates well with the [3H]-thymidine incorporation assay.