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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Alkaline phosphatase (ALP) is a hydrolase enzyme that removes phosphate groups from nucleotides proteins and alkaloids. Changes in ALP level and activity are associated with various disease states in the bone and liver. ALP is also a common enzyme conjugated to secondary antibody used in ELISA.
The Alkaline Phosphatase Activity Fluorometric Assay Kit provides a highly sensitive and convenient way for detection of ALP activity based on fluorometric method in serum and biological samples. In the assay, ALP removes the phosphate group of the non-fluorescent 4-Methylumbelliferyl phosphate disodium salt (MUP) substrate producing an intense fluorescent signal (Ex/Em = 360 nm/440 nm). The kit is an ultra sensitive, HTS-ready assay that is more sensitive than colorimetric method with detection sensitivity ~1 µU. The assay is suited for both research and drug discovery.