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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The Mitochondria/Cytosol Fractionation Kit utilize a special formulation of reagents to isolate enriched mitochondrial fraction from cytosolic fraction of both apoptotic and nonapoptotic mammalian cells. The enriched mitochondrial and cytosolic fraction can be used to detect translocation of factors in apoptosis and signaling pathways with Western blotting, ELISA and other assays etc. The protocol is simple to perform without unltracentrifugations and toxic chemicals.