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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Lactate is an important energy source for living organisms and can generate cellular ATP. Lactate is chiral: L(+)-Lactate and D(-)-Lactate. L(+)-Lactate exists in blood and is constantly produced from pyruvate by lactate dehydrogenase (LDH) in human intermediary metabolism. D(-)-Lactate is present only at about 1-5% of the concentration of L(+)-Lactate. Abnormal high concentration of lactate is related to diseases such as lactate acidosis and diabetes.
The Lactate Fluorometric Assay Kit provides a highly sensitive, simple and convenient way for detection of very low levels of L(+)-lactate in various biological samples based on fluorometric method. In the assay, L(+)-lactate is specifically oxidized to produce an intermediate that reacts with a colorless probe to yield fluorescence (Ex/Em = 535/587 nm), which is directly proportional to the amount of lactate. The kit is suited for high-throughput assay and can detect less than 0.2 µM L(+)-lactate in various biological samples.