Cell Stimulation Cocktail (Without Protein Transport Inhibitors, 500X)

Cell Stimulation Cocktail (Without Protein Transport Inhibitor, 500X) is a pre-mixture cocktail of 12-myristate 13-acetate (PMA) and ionomycin. Stimulation of cells with PMA and ionomycin can induce many cell activations, which in turn induce the production of cytokines. It can then be detected by immunoassays. This cocktail can be combined with protein transport inhibitors (such as brefeldin A and monensin), which allows the resulting cytokines to remain in the cell.

This reagent is supplied as a stock solution dissolved in ethanol and can be used directly. The concentration of PMA is 40.5 μM (25 μg/mL), and ionomycin is 670 μM (0.5 mg/mL).