Rhodamine 123 mitochondrial Membrane Potential Assay Kit
Rhodamine 123 mitochondrial membrane potential assay kit supplies the fluorescent probe Rhodamine 123 for the detection of mitochondrial membrane potential. This kit can be used for membrane potential detection of cellular mitochondria, tissue mitochondria, or purified mitochondria.
Mitochondrial membrane potential, also called ΔΨm, is an important parameter of mitochondrial function and has been used as an important marker of cell apoptosis. Rhodamine 123 is a widely used mitochondrial membrane potential detection probe. Normally, Rhodamine 123 can enter mitochondria in a membrane potential-dependent manner and emit bright yellow-green fluorescence; when the mitochondrial membrane potential of cells decreases, Rhodamine 123 is released from the mitochondria, causing the yellow-green fluorescence in the mitochondria to weaken or disappear. The membrane potential change of mitochondria can be judged by the intensity of Rhodamine 123 fluorescence.
This kit provides CCCP as a positive control. For most cells, the mitochondrial membrane potential almost disappears completely after CCCP treatment. For 6-well plates, this kit can detect 100 samples; For 96-well plates, the kit can detect 1000 samples.
Figure 1: The efficiency of this kit for detection of mitochondrial membrane potential
| Components | K2232-100 T |
| Rhodamine 123 (1000X) | 100 µL |
| Dilution buffer | 100 mL |
| CCCP (10 mM) | 20 µL |
This kit should be stored at -20°C, stable for 1 year. Rhodamine 123 (1000X) and CCCP (10 mM) should be stored at -20°C away from light, and avoid repeated freeze/thaw cycles. | |