SRB Cell Proliferation and Cytotoxicity Assay Kit
SRB Cell Proliferation and Cytotoxicity Assay Kit is a kit that uses sulforhodamine B (SRB) to measure cell viability and toxicity. The detection principle of this kit is based on that SRB dyes can bind to the basic amino acids of proteins in cells and form stable complexes. The complex has specific absorption at 515 nm, and its absorbance is proportional to the number of cells.
Compared with traditional MTT assays, this kit is not only easier to use, but also more sensitive, so it is widely used in cytotoxicity testing, drug screening, and monitoring of cell proliferation and viability.
Figure 1: Reference standard curve for this kit
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Components |
500 Tests |
Storage |
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Fixation Solution |
25 mL |
4°C |
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Wash buffer I |
100 mL |
4°C |
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SRB Staining Solution |
25 mL |
-20°C away from light |
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Wash buffer II (2×) |
500 mL |
4°C |
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Solubilization Solution |
50 mL |
4°C |
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Doxorubicin (20 mM) |
100 μL |
-20°C |
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Shipping: Blue ice Shelf life: 18 months |
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