Cell Mitochondria Isolation Kit I

Cell Mitochondria Isolation Kit I is designed for the rapid and convenient isolation of mitochondria from cultured cells. The mitochondria isolated using this kit are of high purity and mostly maintain the integrity of their inner and outer membrane structures, retaining the physiological functions of mitochondria. This makes them suitable for studies on mitochondrial physiology and protein analysis. The kit also provides Mitochondria Lysis Solution, which can be used to lyse the isolated mitochondria for subsequent experiments such as SDS-PAGE, Western Blot, and two-dimensional electrophoresis. Additionally, to ensure the quality of the isolated mitochondria, the kit includes Trypan Blue Staining Solution, which can be used to assess the degree of homogenization during the mitochondrial preparation process.

This kit can also obtain cytosolic proteins devoid of mitochondria, which can be collected for studying the release of mitochondrial proteins such as cytochrome c into the cytoplasm. Typically, this kit can be used for the extraction of 50-100 samples.