Mito-Tracker Red CMXRos

Mito-Tracker Red CMXRos, also called MitoMark Red I, is an oxidized red fluorescent probe that selectively labels mitochondria. Some conventional fluorescent probes for mitochondria such as tetramethylrosamine and rhodamine 123, are sequestered by functional mitochondria. These probes are easily washed out of cells upon loss in the mitochondria membrane potential. This characteristic limits the use in experiments that require cells to be treated with fixatives or other regent that affect the function of the mitochondria. Mito-Tracker Red CMXRos is a derivative of X-rosamine, which passively diffuses across the plasma membrane of live cells and accumulates in active mitochondria. Meanwhile, Mito-Tracker Red CMXRos is retained in the mitochondria if the cells need fixation after the mitochondria staining.