Fluorometric Proteasome 20S Activity Assay Kit

Proteasomal degradation is one of the main pathways of intracellular protein degradation, which is involved in cellular processes such as apoptosis, gene expression regulation, and oxidative stress, and is closely related to certain cancers, diabetes, and Alzheimer's diseases. Therefore, the proteasome is an important target for drug discovery. The most common proteasome is the proteasome 26S, which contains a 20S core particle structure and two 19S regulatory caps. The 20S core includes three main proteolytic activities, including chymotrypsin-like, trypsin-like, and caspase-like activities.

The Fluorometric Proteasome 20S Activity Assay Kit provides a homogeneous assay for the detection of proteasome 20S activity by the fluorescent substrate LLVY-R110. The proteasome can cleave the LLVY-R110 substrate to produce R110, which emits intensely green fluorescence. Its fluorescence intensity is positively correlated with the activity of the proteasome. The kit can detect cells, pure proteasomes, or cell lysates, and is robust enough for high-throughput screening of proteasome inhibitors.

Figure 1: Detection of Proteasome Activity in Hela cells
The 20S proteasome activity of Hela cells is significantly reduced after treatment with a high concentration of hydrogen peroxide (50 mM H₂O₂).