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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell Stimulation Cocktail (Protein Transport Inhibitors Plus, 500X) is a pre-mixture cocktail of 12-myristate 13-acetate (PMA), ionomycin, brefeldin A, and monensin. Stimulation of cells with PMA and ionomycin can induce many cell activations, which in turn induce the production of cytokines. Brefeldin A and monensin are protein transport inhibitors that allow secreted proteins to accumulate in the endoplasmic reticulum and Golgi, allowing the resulting cytokines to remain in the cell. It can then be detected by immunoassays.
This reagent is supplied as a stock solution dissolved in ethanol and can be used directly. The concentration of PMA is 40.5 μM (25 μg/mL), ionomycin is 670 μM (0.5 mg/mL), brefeldin A is 5.3 mM (1.5 mg/mL), and monensin is 1 mM (0.7 mg/mL).
Components
K2715-100 μL
Cell Stimulation Cocktail (Protein Transport Inhibitors Plus, 500X)
100 μL
Store the reagent at -20°C away from light, stable for 1 year.