HyperTrap Ni-NTA FF Column
HyperChrom Ni-NTA FF Agarose is an affinity chromatography medium made by chemically coupling the transition metal ion Ni2+ with tetracoordination to a ligand of Nitrilotriacetic acid (NTA) using a highly crosslinked 6% agarose gel as a matrix. It has a very stable octahedral structure, nickel ions are at the center of the octahedron, which protects nickel ions from attack by small molecules, is more stable, and can withstand harsh conditions such as reducing agents, denaturants or couplants in a certain concentration. In addition, due to the pressure resistance of the matrix, which can withstand pressures up to 0.3 MPa, the product can be used for industrial large-scale protein purification, enabling purification of the protein of interest at relatively high flow rates. This chromatography medium uses the interaction between Ni2+ and the side chains of certain amino acids (mainly histidine) on the protein to achieve the separation and purification of proteins containing and not containing these amino acids and containing a large and low number of these amino acids. HyperChrom Ni-NTA FF Agarose is the first choice for purifying histidine-tagged proteins, with the advantages of high load, easy regeneration, and low cost.
The HyperTrap Ni-NTA FF Column is a ready-to-use chromatography column with HyperChrom Ni-NTA FF Agarose as the packing material. The main body and inner plug of the pre-installed column tube are made of PP, and the inside and outside are polished, and the sieve plate is made of HDPE, which has excellent physical properties and chemical resistance, corrosion resistance, anti-aging, long life, and good safety. At the same time, it has 1) ready-to-use, convenient and fast; 2) Can be used with syringes, peristaltic pumps or chromatography systems; 3) It can be used in series to increase the sample processing capacity and other advantages. The preloaded column has a standard interface and can be adapted to various commercial medium pressure chromatography systems, such as ÄKTA, which is convenient for customers to purify His-tag recombinant proteins.
- 1. HyperTrap Ni-NTA FF Column preloaded column parameters
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Pre-assembled column specification/name |
1 mL |
5 mL |
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Dimensions (IDxH) |
7.7 x 25 mm |
16 x 25 mm |
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Withstand pressure |
0.3 MPa |
0.3 MPa |
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Workflow Speed* |
1 mL/min |
1-3 mL/min |
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Operating temperature |
4 - 30°C |
4 - 30°C |
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*This flow rate is the recommended common flow rate, the pressure resistance, and the use of different types of chromatography packing in the pre-assembled column will be different, please refer to the product manual of the corresponding chromatographic filler. |
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- 2. HyperChrom Ni-NTA FF Agarose chromatography media parameters
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Name |
Description |
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Chromatography media type |
Affinity chromatography media |
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Ligation |
NTA-Ni2+ |
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Scaffolding |
6% highly cross-linked agarose |
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Average particle size |
90 μm |
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Ligand density |
~15 μmol Ni2+/mL chromatography medium |
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Dynamic load |
≥40 mg histidine-tagged protein/mL chromatography medium* |
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Flow rates are recommended |
90-300 cm/h |
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Maximum flow rate |
400 cm/h |
|
Withstand pressure |
0.3 MPa |
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Use temperature |
4 - 30°C |
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pH stability ** |
2-14 |
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Chemical resistance |
Common aqueous solution, 0.01 M HCl, 0.1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol *** |
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Notes: * Dynamic load measurement conditions: column loading height: 10 cm, test flow rate 150 cm/h; test buffer: 0.02 M PB, 0.5M NaCl, 5 mM imidazole, pH 7.4; test sample: tagged protein (1mg/ml, 6*His); index: protein loading amount per unit media volume (mL) when the protein penetration reaches 10%. **After 7 days of storage of chromatography medium at 40°C and pH 2-14 environment, its physicochemical properties and functions did not change significantly. *** 30% is v/v, volume ratio. |
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| Components | PC1001-1 mL | PC1001-5*1 mL | PC1001-5 mL | PC1001-5*5 mL |
| HyperTrap Ni-NTA FF Column | 1 mL | 5*1 mL | 5 mL | 5*5 mL |
Store the components at 4℃ for 5 years. | ||||