Concanavalin A (Con A)-HyperFluor™ 488
Concanavalin A (Con A), derived from Canavalia ensiformis (Concanavalin), is a plant lectin protein (Mw = 104 kDa) that binds one Ca2+ and one Mn2+ per subunit with a sugar-binding site, and when the metal ions bind to Con A, it can bind various glycoproteins, glycolipids, and α-D-glucose and a-D-mannose moieties in sugars, and HyperFluor™ 488 is a fluorescent dye that fluoresces green (Ex/Em: 495 nm/519 nm) under ultraviolet light. This combination results in a high visualization of concomitant molecules on the cell surface with Con A-HyperFluor™ 488. Concanavalin A-HyperFluor™ 488 is a fluorescently labeled protein that combines Concanavalin A and HyperFluor™ 488 and is widely used in biological research, especially in the field of flow cytometry.
This product is HyperFluor™ 488 conjugated concanavalin A, which is in solution form, which can be used in immunofluorescence and glycobiology research, such as as as being used as a probe to detect carbohydrate molecules on the cell surface, because of its ability to bind to a variety of sugars, it can bind to the cell surface expressing a variety of sugars, and can be detected and analyzed by flow cytometry. It is used for immunohistochemical staining to observe the distribution of sugars in tissues or cells by fluorescence microscopy.