Cy3-UTP
Catalog No.
B8330
used as a substrate for T7 RNA polymerase and generate labeled probes through in vitro transcription
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Cy3-UTP (Cyanine 3-UTP) can replace UTP as a substrate for T7 RNA polymerase and generate labeled probes through in vitro transcription. The resulting assembled mRNA will emit orange fluorescence with the involvement of Cy3-UTPs. In addition, Cy3-labeled mRNA is readily visible under ultraviolet light without further staining after gel electrophoresis[1].
Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .
Reference:
[1]Kim, H., Park, Y., & Lee, J. B. (2015). Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression. Scientific Reports, 5(1). doi:10.1038/srep12737
- 1. Michael I. Gordon, Cathleen Ma, et al. "Double-stranded RNAs targeting Sphaerulina musiva silence DCL but not CYP51 in vitro, and fail to confer resistance to canker in transgenic poplar." PhytoFrontiers 13 Nov 2024
- 2. Sebastian Coupe, Nikta Fakhri, et al. "Nonequilibrium phases of a biomolecular condensate facilitated by enzyme activity." bioRxiv. 2024 Aug 11:2024.08.11.607499 PMID: 39149291
- 3. Kaiqiang Hao, Xinran Gao, et al. "A nanomaterial for the delivery of dsRNA as a strategy to alleviate viral infections in maize." Chemical Engineering Journal Volume 488, 15 May 2024, 150923
- 4. Zhenzhao Sun, Ming Wang, et al. "Getah virus capsid protein undergoes co-condensation with viral genomic RNA to facilitate virion assembly." Int J Biol Macromol. 2024 Apr;265(Pt 1):130847. PMID: 38490381
- 5. Jieli Wei, Yize Zhang, et al. "Multivalent 28S rRNA Is the Organizer of the Nucleolus’s Multi-layered Architecture." bioRxiv. March 14, 2024.
- 6. Meng Xu, Dulmi Senanayaka, et al. "TERRA-LSD1 phase separation promotes R-loop formation for telomere maintenance in ALT cancer cells." Nat Commun. 2024 Mar 9;15(1):2165. PMID: 38461301
- 7. Yan Wang, Yi Guo, et al. "RNA interference-based exogenous double-stranded RNAs confer resistance to Rhizoctonia solani AG-3 on Nicotiana tabacum." est Manag Sci. 2024 Apr;80(4):2170-2178. PMID: 38284497
- 8. Binbin Zheng, Yanping Chen, et al. "Modulating the tumoral SPARC content to enhance albumin-based drug delivery for cancer therapy." J Control Release. 2024 Feb:366:596-610. PMID: 38184232
- 9. Lin Wu, Dian Chen, et al. "A transient conformation facilitates ligand binding to the adenine riboswitch." iScience. PMID: 34927032
| Physical Appearance | Solution |
| Storage | Store at -70°C or below |
| M.Wt | 1151.98 (free acid) |
| Formula | C43H56N5O22P3S2(free acid) |
| Synonyms | Aminoallyl-UTP - Cy3;5-(3-Aminoallyl)-uridine-5'-triphosphate; labeled with Cy3;Triethylammonium salt;Cyanine 3-AA-UTP;Cyanine 3-Aminoallyluridine-5'-Triphosphate;Cyanine 3-UTP |
| Solubility | Soluble in H2O |
| Chemical Name | 2-((1E,3E)-3-(1-(6-(((E)-3-(1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(((hydroxy((hydroxy(phosphonooxy)phosphoryl)oxy)phosphoryl)oxy)methyl)tetrahydrofuran-2-yl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)allyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-ylid |
| SDF | Download SDF |
| Canonical SMILES | O=S(C1=CC=C([N+](CC)=C(C2(C)C)/C=C/C=C(C(C)(C)C3=C4C=CC(S(O)(=O)=O)=C3)/N4CCCCCC(NC/C=C/C5=CN([C@H]6[C@H](O)[C@H](O)[C@@H](COP(OP(O)(OP(O)(O)=O)=O)(O)=O)O6)C(NC5=O)=O)=O)C2=C1)([O-])=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
- View current batch:
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Purity = 95.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
