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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Alkyne-modified biomolecules in mixtures of water with organic solvents can be labeled by Cy5 azide.
Cy5/BHQ2, which is widely used in the research, combinations on several TaqMan probes with various degree of stability of secondary structures and provides some advice on the applicability of dye/quencher pairs for DNA dual labeled probes [1].
Reference:[1] Farzan, V. M.; Aparin, I.O.; Veselova, O.A.; Podkolzin, A.T.; Shipulin, G.A.; Korshun, V.A.; Zatsepin, T.S. Cy5/BHQ dye-quencher pairs in fluorogenic qPCR probes: effects of charge and hydrophobicity. Analytical Methods, in press.