Dextran sulfate sodium salt (DSS)
The dextran sulfate sodium salt form makes it soluble and stable in water. Dextran sulfate contains approximately 17% sulfur, which corresponds to approximately 2.3 sulfate groups per glucose residue. Dextran sulfate sodium salt (DSS) is a polyanionic derivative of dextran formed by esterification of dextran and chlorosulfonic acid.
Inflammatory bowel disease (IBD) is a chronic, relapsing gastrointestinal infection that increases the risk of intestinal tumors. In 1985, it first reported that dextran sodium salt (DSS) could be used to model hamster ulcerative colitis. A large number of studies have shown that the DSS colitis model is similar to human ulcerative colitis. Acute, chronic, and relapsing models of IBD can be induced by altering the concentration of DDS and the frequency of dosing.
Dextran sulfate sodium salt is also used in molecular biology applications, including lipoprotein precipitation, acceleration of DNA probe hybridization with nucleic acids, and ribosome separation.
Reference:
1. Burstein, M., Scholnick, H.R., and Morfin, R. Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions. J. Lipid Res. 11(6), 583-595 (1970).
2. Wahl, G.M., Stern, M., and Stark, G.R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate Proc. Natl. Acad. Sci. U.S.A. 76(8), 3683-3687 (1979).
3. Ascione, R., and Arlinghaus, R.B. Characterization and cell-free activity of polyribosomes isolated from baby hamster kidney cells Biochim Biophys. Acta. 204(2), 478-488 (1970).
- 1. Meng Guo, Rongrong Wang, et al. "Human TFF2-Fc fusion protein alleviates DSS-induced ulcerative colitis in C57BL/6 mice by promoting intestinal epithelial cells repair and inhibiting macrophage inflammation." Inflammopharmacology. 2023 Jun;31(3):1387-1404. PMID: 37129719
- 2. Chen L. "SCCA1/SERPINB3 promotes suppressive immune environment via STAT-dependent chemokine production, blunting the therapy-induced T cell responses." bioRxiv 2023 Feb 03; PMID: 36778224
- 3. Zheng W, Chang IC, et al. "Mycobacterium tuberculosis resides in lysosome-poor monocyte-derived lung cells during persistent infection." bioRxiv 2023 Jan 20 PMID: 36711606
- 4. Alastair Copland, Gillian M. Mackie, et al. "Salmonella cancer therapy metabolically disrupts tumours at the collateral cost of T cell immunity." bioRxiv. January 13, 2023
- 5. Han Li, Bo Pang, et al. "Dioscin promotes autophagy by regulating the AMPK-mTOR pathway in ulcerative colitis." Immunopharmacol Immunotoxicol. 2022 Apr;44(2):238-246. PMID: 35174751
| Physical Appearance | A solid |
| Storage | Store at RT |
| M.Wt | 36000-50000 |
| Cas No. | 9011-18-1 |
| Formula | (C6H7Na3O14S3)n |
| Solubility | insoluble in EtOH; insoluble in DMSO; ≥55.5 mg/mL in H2O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Animal experiment:[1] | |
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Animal models |
Eight-week-old C57BL/6J mice |
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Dosage form |
2.5% DDS in drinking water Administered orally for 7 days |
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Applications |
Oral administration of DDS induced colonic inflammation in a mouse model of inflammatory bowel disease (IBD). Among various chemically induced colitis models, the dextran sulfate sodium-induced colitis model is widely used because of its simplicity and many similarities with human ulcerative colitis. Acute, chronic, and relapsing models of IBD can be achieved by varying the concentration and administration frequency of DDS. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Chassaing B, Aitken JD, Malleshappa M, et al. Dextran sulfate sodium (DSS)-induced colitis in mice. Current Protocols in Immunology, 2014, 104: 15.25.1-15.25.14. |
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Quality Control & MSDS
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