Digoxigenin-11-UTP has been used for labeling RNA probes for in situ hybridization and other purposes. DIG-11-UTP is a substrate for T7, SP6 and T3 RNA polymerases* and can replace UTP in in vitro transcription for DIG-labeling of RNA in a ratio of 35%: 65%.
Linearized template DNA with T7, SP6 or T3 promoter is in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP and DIG-11-UTP, respectively.
Labeled RNA can be subsequently detected with the DIG Luminescent Detection Kit for Nucleic Acids* or anti-DIG-AP* and CDP-Star*.
References:
[1] Mugford S, et al., A Serine Carboxypeptidase-Like Acyltransferase Is Required for Synthesis of Antimicrobial Compounds and Disease Resistance in Oats, The Plant Cell 21:2473-2484 (2009)
[2]-Pontes O,et al., Natural variation in nucleolar dominance reveals the relationship between nucleolus organizer chromatin topology and rRNA gene transcription in Arabidopsis, PNAS vol. 100 no. 20 11418-11423 (2003)
[3]Schaeren-Wiemers N.et al., A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes, Histochemistry and Cell Biology, Volume 100, Number 6 (1993)
[4]Yanyan L. et al., PiggyBac transgenic strategies in the developing chicken spinal cord, Nucleic Acids Res., Sep 2009;10.1093/nar/gkp686
[5]Zwirglmaier K.et al., In situ functional gene analysis: recognition of individual genes by fluorescence in situ hybridization, Methods Enzymol. 397:338-51 (2005)