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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
DiO, also called DiOC18(3), is a green fluorescent probe that is widely used as a lipophilic tracker in living and fixed tissues and cells. DiO can insert into the membrane, and diffuse rapidly, staining the entire membrane surface green. DiO usually does not affect cell viability and development, so it is commonly used in cell tracking, such as anterograde and retrograde neuronal tracking. DiO staining is usually less intense than DiI staining, and occasionally fails completely in fixed tissues.
DiO staining can be used in conjunction with immunofluorescence. It is recommended to use formaldehyde (PFA) for fixation after DiO staining. Meanwhile, if permeabilization is required, it is suggested to use Triton X-100 or digitonin. But permeabilization may affect the localization of DiO in the cell membrane. In addition, DiO has many other applications including detecting cell-cell fusion and adhesion, tracking cell migration, and labeling lipoproteins.
The product should be stored at -20°C away from light and moisture. Stable for 1 year. The stock solution should be stored at -20°C away from light. Stable for 6 months.
