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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
DL-threo-2-methylisocitrate sodium is a substrate of isocitratelyase 1(ICL1).
ICL1 had dual activity for both threo-D(s)L(s)-isocitrate (ICA) and DL-threo-2-methylisocitrate (MICA). Using Michaelis-Menten non-linear least squares fit, the Km of purified recombinant ICL1 for threo-D(s)L(s)-isocitrate (ICA) was determined to be 188 mM with akcatof 5.24 s-1 .The Km of ICL2 for MICA was greater than 15 mM which was too high to be determained accurately due to the limitations of the solubility of MICA. The Kcat/Km for ICL1, a measureforcatalyticefficiency, was 2.79 × 104 M-1 s-1 and 1.74 ×103 M-1 s-1, for ICA and MICA respectively.
Reference:[1]. Gould TA1,van de Langemheen H,Muoz-Elías EJ,McKinney JD,Sacchettini JC. Dual role of isocitratelyase 1 in the glyoxylate and methylcitrate cycles in Mycobacterium tuberculosis.Mol Microbiol.2006 Aug;61(4):940-7.