DNase I (RNase-free)
DNase I (RNase-free) is an endonuclease that digests single- or double- stranded DNA, generating dinucleotide, trinucleotide, and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. The activity of DNase I (RNase-free) dependent on Ca2+ and can be activated by Mg2+ or Mn2+. In the presence of Mg2+, DNase I (RNase-free) can randomly cleaves arbitrary sites of double-stranded DNA; in the presence of Mn2+, DNase I (RNase-free) can simultaneously recognize both strands of DNA and cleaves at nearly same sites.
DNase I (RNase-free) can act on single stranded DNA, double stranded DNA, chromatin, and RNA: DNA hybrid strands. DNase I (RNase-free) is suitable for RNA extraction, in vitro transcription, and removal of DNA for RT-PCR experiments.
| Components | 1000 U | 5000 U | 10000 U |
| DNase I (RNase-free) (2 U/μl) | 500 μl | 500 μl x 5 | 1 ml x 5 |
| 10X DNase I Buffer | 1.5 ml | 1.5 ml | 1.5 ml x 2 |
Store the components at -20°C. | |||