Dpn I
Catalog No.
K1104
Restriction endonuclease, sensitive to Dam methylation
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Dpn I is a Dam methylation-sensitive restriction enzyme that recognizes GA/TC sequences, and can only cleave DNA when adenine N6-methylation is present in the sequence. The digestion will be blocked when CpG methylation of mammalian DNA overlaps with restriction sites of Dpn I. In addition, Dpn Ⅰ had no effect on Dcm methylation. This product is suitable for rapid digestion reactions of plasmid DNA, PCR products or genomic DNA, and is widely used in many research fields such as genotyping, molecular cloning, Southern blotting, SNP, RFLP technology, etc.
The restriction sites of Dpn I as follows:
5'…… G Am6 ↓T C…… 3'
3'…… C T↑ Am6G…… 5'
| Components | 500 U | 1000 U | 5000 U |
| Dpn I (20 U/μL) | 0.025 mL | 0.05 mL | 0.25 mL |
| 10X Dpn I Reaction Buffer | 0.5 mL | 1 mL | 5 mL |
Store the components at -20 °C. | |||