Dual Luciferase Assay System

The dual luciferase reporter gene system is one of the important means to study the regulatory expression of genes. This kit contains high-purity firefly Luciferin and Coelenterazine, which emit bioluminescence of the corresponding wavelength during the reaction, where the firefly fluorescein emits a yellow-green light with a wavelength of 550-570 nm in the presence of firefly luciferase, oxygen, ATP and magnesium ions at the same time, while Coelenterazine can react to emit a blue light with a wavelength of 480 nm in the presence of Renilla luciferase and oxygen.

In the experiment, the expression of firefly Luciferase (Firefly Luciferase) was detected by fluorescein as a substrate, and then the fluorescence signal of firefly luciferase was quenched, and the expression of Renilla luciferase was detected with luciferin as the substrate to achieve dual luciferase reporter gene detection.

This product does not need to lyse the cells with lysate in advance, and then add fluorescence detection reagents, simplifying the experimental process. Luciferase reagent is added directly to the cell culture when used, the cells are lysed, and a firefly luciferase substrate is provided at the same time, which makes this product more suitable for high-throughput detection. Our reagents are suitable for mammalian cell culture media, such as the following types of media containing 1-10% serum: RPMI 1640, DMEM, MEMα and F12, etc.