FITC, Fluorescein isothiocyanate
FITC, also known as fluorescein 5-isothiocyanate, is a fluorescein derivative used in a variety of applications including flow cytometry. FITC reacts with amine groups to form conjugates with proteins for detection by various fluorescent-based applications. The excitation and emission maxima of FITC are 494 and 519 nm, respectively. Therefore, FITC serves as a useful reagent for attaching the fluorescein chromophore to amino groups of proteins, for the purpose of the micro-detection of protein in cells under ultraviolet light. In addition, FITC has also been proven to be a safe and effective technique for lymphatic mapping in the Lymphatic Microsurgical Preventing Healing Approach (LYMPHA) procedure.
References:
1. Sabnis RW. Fluoroscein-5-isothiocyanate (FITC). Handbook of Fluorescent Dyes and Probes, 2015, 219 - 223.
2. Maeda H, Ishida N, Kawauchi H, et al. Reaction of fluorescein-isothiocyanate with proteins and amino acids. I. Covalent and non-covalent binding of fluorescein-isothiocyanate and fluorescein to proteins. Journal of Biochemistry, 1969, 65(5): 777-783.
3. Spiguel L, Shaw C, Katz A, et al. Fluorescein isothiocyanate: a novel application for lymphatic surgery. Annals of Plastic Surgery, 2017, 78(6S Suppl 5): S296-S298.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 389.38 |
| Cas No. | 3326-32-7 |
| Formula | C21H11NO5S |
| Solubility | ≥38.9 mg/mL in DMSO; insoluble in H2O; ≥21.2 mg/mL in EtOH |
| Chemical Name | 3',6'-dihydroxy-5-isothiocyanato-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one |
| SDF | Download SDF |
| Canonical SMILES | S=C=NC1=CC(C(OC23C4=C(OC5=C3C=CC(O)=C5)C=C(O)C=C4)=O)=C2C=C1 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
