Flt3-Ligand, human recombinant
Catalog No.
P1010
Flt3-ligand, expressed in E.Coli, stimulate the proliferation of hematopoietic cells.
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Human FMS-like tyrosine kinase 3 ligand (Flt3-ligand) is a cytokine that synergizes with other CSFs and interleukins to induce proliferation and differentiation of early hematopoietic cells. Human Flt3-ligand cDNA encodes a 235 amino acid residue precursor protein that is cleaved to yield mature human Flt-3 ligand, which consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Recombinant human flt3-ligand is a soluble 17.6 kDa polypeptide containing 155 amino acid residues, which comprises the extracellular domain of the transmembrane flt3-ligand protein.
| Determined by the dose-dependent stimulation of the proliferation of human AML5 cells. | ||||||
| ED50 | < 0.1 ng/ml. | |||||
| Specific activity | > 1*106 units/mg. | |||||
Quality Control & DataSheet
- View current batch:
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Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level:<0.1 ng/μg
Related Biological Data
| Gene ID: | 2323 | Accession #: | P49771 |
| Alternate Names: | Flt3-L, human flt3-Ligand, flt3-Ligand, flt3Ligand, flt3 Ligand,h-flt3-Ligand, rh-flt3-Ligand, recombinant human flt3-Ligand, recombinant flt3-Ligand. | ||
| Source: | Escherichia Coli. | M.Wt: | 17.6 kDa |
| Appearance: | Lyophilized protein | Storage: | Store at -20°C |
| Formulation: | Sterile filtered and lyophilized with no additives. The protein may appear as a haze of film, which is difficult to see at the bottom of the vial. | ||
| Solubility: | Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 week or -20℃ for future use. | ||
| Shipping Condition: | gel pack. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||