Folinic acid
Folinic acid, clinically known as leucovorin, is a ubiquitous member of biological folate pools, usually accounting for 10 ~ 20% of total cellular folates. Folinic acid can reduce methotrexate (MTX)-induced cytogenetic damage, and appears to be an anticlastogenic agent. Folinic acid serves as a rescue agent used alone or in combination with 5-fluorouracil to decrease MTX-induced toxicity.
References:
1. Keshava C, Keshava N, Whong WZ, et al. Inhibition of methotrexate-induced chromosomal damage by folinic acid in V79 cells. Mutation Research, 1998, 397(2): 221-228.
2. Iqbal MP, Ahmed M, Umer M, et al. Effect of methotrexate and folinic acid on skeletal growth in mice. Acta Paediatrica, 2003, 92(12): 1438-1444.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 473.44 |
| Cas No. | 58-05-9 |
| Formula | C20H23N7O7 |
| Solubility | ≥7.62 mg/mL in DMSO with gentle warming; insoluble in H2O; insoluble in EtOH |
| Chemical Name | (2S)-2-(4-(((5-formyl-4-hydroxy-2-imino-1,2,5,6,7,8-hexahydropteridin-6-yl)methyl)amino)benzamido)pentanedioic acid |
| SDF | Download SDF |
| Canonical SMILES | N=C1NC(NCC(N2C=O)([H])CNC3=CC=C(C(N[C@@](C(O)=O)([H])CCC(O)=O)=O)C=C3)=C2C(O)=N1 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
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Cell lines |
V79 cells |
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Reaction Conditions |
5 or 50 μg/ml folinic acid for 40 h incubation |
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Applications |
MTX alone induced a concentration-related increase in the percentages of micronucleated binucleated cells (MNBN) and aberrant cells (Abs). The addition of folinic acid at 50 μg/ml significantly reduced MNBN by 40 ~ 68% and Abs by 36 ~ 77%. Inhibition was also seen at 5 μg/ml folinic acid (12 to 54% for MNBN and 20 to 61% for Abs). |
| Animal experiment:[2] | |
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Animal models |
Balb/c young growing male mice, 11.9 ± 0.25 g |
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Dosage form |
7.0 mg/kg Injected intraperitoneally every second day for 3 weeks |
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Applications |
Chronic administration of MTX induced suppression of skeletal growth in mice. Folinic acid treatment following MTX administration appeared to reverse this growth inhibition. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Keshava C, Keshava N, Whong WZ, et al. Inhibition of methotrexate-induced chromosomal damage by folinic acid in V79 cells. Mutation Research, 1998, 397(2): 221-228. 2. Iqbal MP, Ahmed M, Umer M, et al. Effect of methotrexate and folinic acid on skeletal growth in mice. Acta Paediatrica, 2003, 92(12): 1438-1444. |
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Quality Control & MSDS
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Chemical structure
