His-tag Protein Purification Kit (Non-Denaturant)

His-tagged protein purification kit is a tool for the extraction of His-tagged recombinant proteins from cells or cell lysates. These kits typically contain affinity chromatography media, such as Ni-Agarose or Ni-NTA resins, that specifically bind to histidine residues on the His-tag. When using the His-tagged protein purification kit, the protein sample is passed through an affinity chromatography medium, the target protein is adsorbed, while the other proteins are not bound, and the target protein can be eluted off by changing buffer conditions after the impurities are removed by washing.

This product is a denaturable His-tagged protein purification kit, which contains the relevant reagents required for His-tagged proteins and the empty column tube of the affinity chromatography column, which brings great convenience to the purification of His-tagged proteins. This kit provides the lysate, wash, and eluate needed to purify His-tagged proteins under native conditions.

This product is sufficient for 10 purifications of His-tagged recombinant proteins, with a wet weight of approximately 1 gram per bacterial pellet. The maximum amount of protein purified per time (~55 kD) is 12-18 mg, and the specific maximum amount of purification depends on the molecular weight of the protein. Purification of a small amount of His-tagged protein can be performed in 500 sessions without the use of an affinity column empty column tube.