Hoechst 33342/PI Double Staining Kit
Hoechst 33342/PI Double Staining Kit is a kit that can rapidly detect cell apoptosis and necrosis. This kit provides two dyes, Hoechst 33342 and propidium iodide (PI). Hoechst 33342 can penetrate cell membranes, staining normal cells with a weak blue color. Chromatin condensation is a hallmark of apoptosis. Hoechst 33342 can stain condensed chromatin of apoptotic cells more brightly than normal cells. PI cannot penetrate the cell membrane and cannot stain normal cells or apoptotic cells red; for necrotic cells (whose membrane loses integrity), PI can stain them red. When using these two dyes for double staining, normal cells are weak blue + weak red fluorescence, apoptotic cells are strong blue + weak red fluorescence, and necrotic cells are strong blue + strong red fluorescence.
Figure 1: Schematic of this kit to detect the cell apoptosis
Normal cells (Control) are mainly stained with a weak blue color by Hoechst 33342; When cells are induced to undergo apoptosis, apoptotic cells (Apoptosis) are mainly stained with strong blue by Hoechst 33342, and necrotic cells are stained red by PI
| Components | K2237-100 T | K2237-500 T |
| Hoechst 33342 staining solution | 250 µL | 1.25 mL |
| PI staining solution | 250 µL | 1.25 mL |
| Staining buffer | 100 mL | 5 x 100 mL |
Store the kit at -20°C, stable for 1 year. Hoechst 33342 staining solution and PI staining solution should be stored away from light. | ||