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HyperChrom AR rProtein A FF Agarose is an affinity chromatography medium for antibody purification, separated by specific interaction between antigen and antibody, mainly used to purify monoclonal and polyclonal antibodies.
HyperChrom AR rProtein A FF Agarose has high selectivity, high yield, and easy to scale up properties: (1) recombinant protein A is produced in E. coli and engineered for directional conjugation to enhance binding load; Epoxy-based conjugation ensures low ligand shedding; recombinant protein A has a specificity similar to native protein A to the LgG Fc region, which improves selectivity and yield. (2) Process amplification is relatively simple.
HyperChrom AR rProtein A FF Agarose chromatography media parameters
Name
Description
Chromatography media type
Affinity chromatography media
Ligation
rProtein A (E. coli recombinant expression)
Scaffolding
Highly cross-linked agarose
Average particle size
90 μm
Ligand density
6 mg rProtein A/ml chromatography medium
Dynamic load
~35 mg human IgG/mL chromatography medium*
Flow rates are recommended
150-500 cm/h
Maximum flow rate
700 cm/h
Withstand pressure
0.3 MPa
Use temperature
4-30℃
pH stability **
3-10
Chemical resistance
6 M guanidine hydrochloride, 8 M urea, 2% benzyl alcohol, 75% ethanol, 1 mM NaOH (pH11), 0.1 M sodium citrate/HCl (pH3).
** Dynamic load measurement conditions: column loading height: 5 cm, retention time 3 min, test buffer: 0.02 M NaH2PO4 solution, pH 7.0, test sample: human lgG sample, lgG sample per unit media volume (ml) when the penetration of lgG reaches 10%.
**After 7 days of storage of chromatography medium at 40°C and pH 3-10, its physicochemical properties and functions did not change significantly.
***30% and 75% are v/v, volume ratio.
Store the components at 4℃ for 5 years.