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HyperChrom Heparin HP Agarose is an affinity chromatography medium made by covalently coupling heparin to an agarose base. Heparin is a naturally occurring glycosaminoglycan that has affinity with a variety of biomolecules and can also be used as an ion exchange ligand. This chromatography medium can be used to isolate and purify coagulation factors, antithrombin III, growth factors, interferon, lipoprotein lipase, and enzymes for nucleic acid and steroid receptors.
Compared to HyperChrom Heparin FF Agarose, HyperChrom Heparin HP Agarose has a finer particle size and provides higher resolution.
HyperChrom Heparin HP Agarose chromatography media parameters
Dame
Description
Chromatography media type
Heparin affinity chromatography mediator
Ligation
heparin
Scaffolding
Highly cross-linked agarose
Average particle size
34 μm
Ligand density
~10 mg/mL chromatography medium
Flow rates are recommended
90-150 cm/h
Maximum flow rate
200 cm/h
Withstand pressure
0.3 MPa
Use temperature
4-30℃
pH stability *
4-12
Chemical resistance
Common aqueous solution, 4 M NaCl, 0.1 M NaOH, 0.05 M sodium acetate (pH4), 6 M guanidine hydrochloride, 8 M urea, 70% ethanol**
* After 7 days of storage of chromatography medium at 40 °C and pH 4-12, its physicochemical properties and functions did not change significantly.
**70% is v/v, volume ratio.
Store the components at 4℃ for 5 years.