HRP Rabbit Anti-Goat IgG (H+L) Antibody
Name | HRP Rabbit Anti-Goat IgG (H+L) Antibody |
Host species | Rabbit |
Target species | Goat |
Tested applications | Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WB |
Conjugation | HRP |
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for Goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Application | Notes |
Dot blot | Use at an assay dependent dilution. |
ELISA | 1/25000-1/50000. |
IHC-P | Use at an assay dependent dilution. |
IHC-Fr | Use at an assay dependent dilution. |
Immunomicroscopy | Use at an assay dependent dilution. |
ICC/IF | 1/500-1/2500. |
WB | 1/2000-1/20000. |
Form | Liquid |
Storage buffer | pH: 7.40 Preservative: 1% BSA,50% glycerol,0.01% proclin 300 Constituent: PBS |
Storage instructions | Shipped at 4°C. Store at 4°C short term (2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. |
Concentration | 1 mg/mL |
Purity | Immunogen affinity purified |
Purification notes | This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. |
Clonality | Polyclonal |
Isotype | IgG |