JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.
Tel: +1-832-696-8203
Email: [email protected]
Worldwide Distributors
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Hydrocortisone is a main glucocorticoid secreted by the adrenal cortex.
Cell lines
human lung microvascular endothelial cells
Preparation method
The solubility of this compound in DMSO is >13.3 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37°C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.
Reacting condition
4 or 6 μM; 16 h
Applications
In human lung microvascular endothelial cells, Hydrocortisone (HC) induced a concentration-dependent but biologically minor barrier enhancing and protective effect. The combination of HC (4 μM) and ascorbic acid (VitC) (1000 μM) pre- or post-treatment significantly reversed the LPS-induced barrier dysfunction.
Animal models
6-hydroxydopamine (6-OHDA)-induced Parkinson’s disease (PD) mouse models
Dosage form
0.4 mg/kg; intraperitoneal injection, 7 consecutive days
Application
In Parkinson’s disease (PD) mouse models, Hydrocortisone caused approximate two-fold increase of parkin expression in selected brain subregions (striatum, ventral midbrain, and cerebellum), which was responsible for cell survival against oxidative stress. Hydrocortisone also increased CREB expression. Hydrocortisone pretreatment markedly enhanced dopaminergic neuronal survival against 6-OHDA intoxication.
Other notes
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References:
[1] Barabutis N1, Khangoora V2, Marik PE2, et al. Hydrocortisone and Ascorbic Acid synergistically prevent and repair LPS-induced pulmonary endothelial barrier dysfunction. Chest. 2017 Jul 21. pii: S0012-3692 (17) 31276-X.
[2] Ham S1, Lee YI2, 3, Jo M1, et al. Hydrocortisone-induced parkin prevents dopaminergic cell death via CREB pathway in Parkinson's disease model. Sci Rep. 2017 Apr 3; 7 (1): 525.