Hyper Assembly Cloning Kit

Hyper Assembly Cloning Kit is designed for fast, directional cloning of one or more target fragment of DNA into any vector. This method employs the 3’-5’ exonuclease activity of Hyper Assembly Enzyme to get 15-20 nt complementary arms at the ends of PCR-generated inserts and linearized vectors, then fuse the DNA fragments to get the recombinant vectors. These 15-20 bp overlaps can be engineered by designing primers for amplification of the desired sequences.

• Clone any insert into any location within any vector you choose

• Efficiently clone a broad range of fragment sizes

• Clone multiple DNA fragments simultaneously into any vector in a single reaction

• No restriction digestion, phosphatase treatment, or ligation required