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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Loop Mediated Isothermal Amplification (LAMP) is a new technique for nucleic acid amplification in vitro. The reaction process is always maintained at a constant temperature, and the rapid expansion of nucleic acid is carried out by adding different active enzymes and their specific primers. The principle of the LAMP Colorimetric Assay Kit is to amplify DNA using LAMP method and to detect the presence of target DNA in a sample by color change of the reaction system. Compared with conventional PCR, LAMP does not require thermal denaturation, temperature cycling, and subsequent electrophoresis and ultraviolet observation of the template, and only needs to incubate at isothermal conditions for 1 h to complete nucleic acid amplification reaction, which has the advantages of simple and rapid, high sensitivity, and strong specificity.
This kit uses dU incorporation and UDG enzyme to prevent contamination of isothermal amplification products, including Bst DNA Polymerase with chain replacement activity. By designing 4-6 specific isothermal amplification primers for different regions of target genes, the target DNA in samples can be detected quickly, effectively and with high sensitivity. And the use of visual color change technology, without electrophoresis, through the naked eye to observe the color change can judge the result, suitable for detecting whether there is a specific pathogen, microbial contamination in the sample.
Components
25 rxns
100 rxns
Storage
2 × LAMP Master Mix with UDG
250 μL
1 mL
-20°C, protect from light
Bst DNA Polymerase
25 μL
100 μL
-20°C
Positive Control
50 μL
200 μL
Mineral Oil
500 μL
2 mL
Nuclease-free Water
Shipping: Dry Ice Shelf life: 12 months