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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperScribeTM T7 High Yield Cy5 RNA Labeling Kit Plus is designed to generate randomly Cy5-modified RNA probes by in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern blot hybridization experiments. The principle of labeling is similar to the basic labeling principle of a mixture of Cy5 RNA labels. Cy5-UTP is efficiently incorporated into RNA using the optimized reaction buffer and T7 RNA polymerase mixture in place of its natural counterpart UTP. An appropriate Cy5-UTP substitution typically achieves an optimal balance between reaction and labeling efficiency. The resulting Cy5-modified RNA probe can then be detected by fluorescence spectroscopy.
This kit adjusts the composition and reaction based on K1062 to achieve higher RNA yields for standard 20 μL reaction systems.
Store the components at -20°C.