HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit
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- 2. Ming Zhao, Yu Yu, et al. "GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein." Nat Commun. 2021 Apr 9;12(1):2114. PMID: 33837182
| Components | 25 rxns |
| T7 RNA Polymerase Mix | 50 uL |
| 10X Reaction Buffer | 50 uL |
| ATP (20 mM) | 50 uL |
| GTP (20 mM) | 50 uL |
| UTP (20 mM) | 37.5 uL |
| CTP (20 mM) | 50 uL |
| Cy5-UTP (10 mM) | 25 uL |
| Control Template (0.5 ug/uL) | 5 uL |
| RNase-free H2O | 1 mL |
Store all the kit components at -20°C. | |
HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit is designed to generate randomly Cy5-modified RNA probes by in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern blot hybridization experiments. The principle of labeling is similar to the basic labeling principle of a mixture of Cy5 RNA labels. Cy5-UTP is efficiently incorporated into RNA using the optimized reaction buffer and T7 RNA polymerase mixture in place of its natural counterpart UTP. An appropriate Cy5-UTP substitution typically achieves an optimal balance between reaction and labeling efficiency. However, the individual optimization of the Cy5-UTP/UTP ratio can be easily achieved using a single nucleotide format. The resulting Cy5 -modified RNA probe can then be detected by fluorescence spectroscopy.
An upgraded version of this product is available with higher yield (~100ug), please check out K1404 HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit Plus for more details.