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Worldwide Distributors
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The HyperScribe™T7 siRNA Synthesis Kit is designed for the rapid production of double-stranded RNA (dsRNA) in vitro. Short siRNA and long dsRNA were synthesized in vitro by using T7 RNA polymerase with linear double-stranded DNA containing T7 Promoter sequence as template and NTPs as substrates. In addition, this kit contains RNase T1, which can specifically cut off the 3 'phosphoric acid of the guanylate of single-stranded RNA. The resulting double-stranded RNA transcripts can be purified for subsequent RNAi related experiments such as lipid nanoparticle (LNP) delivery and microinjection.
This kit comes in two boxes, shipped using dry ice and blue ice respectively.
Components
25 rxns
50 rxns
Storage
T7 RNA polymerase Mix
50 μL
100 μL
-20°C
10 × Reaction Buffer
10 × Annealing Buffer
250 μL
500 μL
NTP Mix
200 μL
400 μL
DNase I
25 μL
RNase T1 (100 U/μL)
RNase T1 Dilution Buffer
300 μL
600 μL
Control Template
5 μL
10 μL
RNA Clean Beads
2 mL
4 mL
4°C
RNase-free H2O
5 mL
10 mL
Shipping: Dry Ice and Blue Ice, Respectively Shelf life: 2 years