HyperScribe™ T7 siRNA Synthesis Kit
The HyperScribe™T7 siRNA Synthesis Kit is designed for the rapid production of double-stranded RNA (dsRNA) in vitro. Short siRNA and long dsRNA were synthesized in vitro by using T7 RNA polymerase with linear double-stranded DNA containing T7 Promoter sequence as template and NTPs as substrates. In addition, this kit contains RNase T1, which can specifically cut off the 3 'phosphoric acid of the guanylate of single-stranded RNA. The resulting double-stranded RNA transcripts can be purified for subsequent RNAi related experiments such as lipid nanoparticle (LNP) delivery and microinjection.
This kit comes in two boxes, shipped using dry ice and blue ice respectively.
Related Biological Data
Related Biological Data
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Components |
25 rxns |
50 rxns |
Storage |
|
T7 RNA polymerase Mix |
50 μL |
100 μL |
-20°C |
|
10 × Reaction Buffer |
50 μL |
100 μL |
-20°C |
|
10 × Annealing Buffer |
250 μL |
500 μL |
-20°C |
|
NTP Mix |
200 μL |
400 μL |
-20°C |
|
DNase I |
25 μL |
50 μL |
-20°C |
|
RNase T1 (100 U/μL) |
25 μL |
50 μL |
-20°C |
|
RNase T1 Dilution Buffer |
300 μL |
600 μL |
-20°C |
|
Control Template |
5 μL |
10 μL |
-20°C |
|
RNA Clean Beads |
2 mL |
4 mL |
4°C |
|
RNase-free H2O |
5 mL |
10 mL |
4°C |
|
Shipping: Dry Ice and Blue Ice, Respectively Shelf life: 2 years |
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