HyperScript™ First-Strand cDNA Synthesis Kit
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The HyperScript™ First-Strand cDNA Synthesis Kit is based on HyperScript™ Reverse Transcriptase (Cat. No. K1071) to synthesize first-strand cDNA from Total RNA or Poly(A)+ RNA. HyperScript™ Reverse Transcriptase is a new enzyme obtained through genetic engineering based on M-MLV (RNase H-) Reverse Transcriptase. In comparison, HyperScript™ Reverse Transcriptase reduces RNase H activity and increases thermal stability. HyperScript™ Reverse Transcriptase can withstand higher reaction temperatures and is suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HyperScript™ Reverse Transcriptase enhances the affinity to the template, suitable for the reverse transcription of a small number of templates and low copy genes, and can generate cDNA up to 12.3 kb.
The HyperScript™ First-Strand cDNA Synthesis Kit contains all the components needed to synthesize the first-strand cDNA and provides two cDNA synthesis primers, Random Primers and Oligo (dT)23VN. You can choose Random Primers, Oligo (dT)23VN or gene-specific primers as reverse transcription primers according to the needs of the experiment. The synthesized first-strand cDNA products can be used in subsequent experiments such as PCR amplification and qPCR reaction.
| Components | 20 rxn(20 μL reaction) | 50 rxn(20 μL reaction) | 100 rxn(20 μL reaction) |
| HyperScript™ Reverse Transcriptase (200 U/μL) | 20 μL | 50 μL | 100 μL |
| 5X First-Strand Buffer | 80 μL | 200 μL | 400 μL |
| RNase Inhibitor, Murine(40 U/μL) | 20 μL | 50 μL | 100 μL |
| 10 mM dNTP Mixture | 20 μL | 50 μL | 100 μL |
| RNase Free ddH2O | 1 mL | 1 mL | 1 mL x 2 |
| Random Primers (50 μM) | 20 μL | 50 μL | 100 μL |
| Oligo (dT) 23VN (50 μM) | 20 μL | 50 μL | 100 μL |
Store the components at -20°C. | |||
Efficient HyperScript™ Reverse Transcriptase for RNA templates containing secondary structures
Synthesis of first-strand cDNA up to 12.3 kb
Detects template starting amounts as low as 1 pg
Oligo (dT)23VN primers have stronger template anchoring ability and higher reverse transcription efficiency than Oligo (dT)18
Synthesized first-strand cDNA can be used for experiments such as PCR amplification and qPCR reaction